Figure 4
Similarity of MtAACT protein to other known AACT proteins and effect of Al3+on expression of MtAACT in J. A17 and R108 plants. Phylogenetic tree of known and putative Al-activated citrate transporters was constructed by MEGA 5 in panel (a). The accession numbers of SbMATE, HvAACT1, AtMATE, ZmMATE1, MtMATE, At4g38380 and At2g38330 in GenBank are ABS89149.1, BAF75822.1, NP_974000.1, ACM47311.1, XP_003627698.1, NP_195551.5 and NP_181367.2, respectively. The expression of MtAACT in roots of J. A17 and R108 plants under the conditions of with or without 5 μM A1Cl3 (pH 4.5) in medium for 1 days (b). Data are mean ± s.e. with three biological replicates. * and ** indicate significant difference between genotypes within a given growth condition (−Al or + Al) at P ≤ 0.05 and P ≤ 0.01, respectively.