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Table 4 Glucosinolate content in different tissues of selected Arabidopsis mutants

From: Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

Mutant (gene)

Tissue

3MSOP

4MSOB

4MTB

5MSOP

8MSOO

I3M

4MOI3M

1MOI3M

Total GS

Atcyp81f4 (At4g37410)

Leaves

92 ± 20

90 ± 14

97 ± 11

104 ± 11

102 ± 19

108 ± 11

98 ± 21

-*

95 ± 11

 

Roots

-

-

-

-

98 ± 15

163 ± 15*

120 ± 21

-*

113 ± 13

Atcyp81f1 (At4g37430)

Leaves

99 ± 3

92 ± 1

123 ± 14

102 ± 4

96 ± 5

129 ± 17

99 ± 6

103 ± 2

104 ± 2

 

Roots

-

-

-

-

93 ± 1

104 ± 2

99 ± 11

79 ± 15

89 ± 2

Atcyp81d1 (At5g36220)

Leaves

101 ± 9

108 ± 1

81 ± 4

98 ± 21

122 ± 16

87 ± 4

101 ± 5

97 ± 31

96 ± 2

 

Roots

-

-

-

-

108 ± 5

96 ± 2

101 ± 7

169 ± 36

112 ± 8

Atomt (At4g35160)

Leaves of flowering plant

76 ± 30

103 ± 43

-

-

-

97 ± 76

7 ± 2*

40 ± 15*

90 ± 47

  1. 3MSOP, 3-methylsulfinyl-propyl GS; 4MSOB, 4-methylsulfinyl-butyl GS; 4MTB, 4-methylthio-butyl GS; 5MSOP, 5-methylsulfinyl-pentyl GS; 8MSOO, 8-methylsulfinyl-octyl GS; I3M, indol-3-ylmethyl GS; 4MOI3M, 4-methoxy-indol-3-ylmethyl GS; 1MOI3M, 1-methoxy-indol-3-ylmethyl GS. Values represent the mean ± standard deviation of three to six individual plants homozygous for the respective T-DNA insertion. Significant differences to the respective control tissue (P < 0.05) as determined using unpaired two-tailed t-test, are marked with an asterisk. Values are given in % on dry matter basis of the respective control tissue. For absolute concentrations of glucosinolates in the respective control tissue please see supporting Additional file 1: Table S3. -, below detection limit.