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Table 6 Semi-quantitative realtime RT-PCR analysis of the selected genes in different tissues of pak choi

From: Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

Abbreviation

Treatment

Seedlings

Leaves

Roots

BrCYP81F1

Control

-9.2 ± 0.64

-3.6 ± 0.68

-11.6 ± 2.76

 

500 μM MeJA

-7.4 ± 0.18

-4.2 ± 2.05

-12.1 ± 1.52

BrCYP81F2

Control

-10.6 ± 0.37

-6.8 ± 1.26

-9.1 ± 0.94

 

500 μM MeJA

-7.7 ± 0.42

-6.9 ± 1.43

-8.2 ± 0.75

BrCYP81F3-1

Control

-6.3 ± 0.23

-3.4 ± 0.48

-6.5 ± 0.57

 

500 μM MeJA

-5.7 ± 0.42

-3.2 ± 0.44

-6.9 ± 0.45

BrCYP81F3-2

Control

-6.5 ± 0.20

-6.4 ± 0.74

-8.9 ± 0.32

 

500 μM MeJA

-5.7 ± 0.20

-5.2 ± 1.41

-6.6 ± 0.41

BrCYP81F4-1

Control

-7.5 ± 0.61

-3.5 ± 0.30

-2.1 ± 0.30

 

500 μM MeJA

-2.4 ± 0.65

0.3 ± 0.96

1.5 ± 0.20

BrCYP81F4-2

Control

-8.5 ± 0.40

-2.3 ± 0.37

-6.2 ± 0.60

 

500 μM MeJA

-0.7 ± 0.75

2.3 ± 0.52

1.4 ± 0.60

BrOMT

Control

-8.3 ± 0.51

-5.5 ± 0.81

-6.7 ± 0.91

 

500 μM MeJA

-3.0 ± 0.99

nd

nd

  1. Each value represents the Ct value relative to that of Actin and is given as mean ± standard deviation of four individual samples. Measurements were repeated twice. Methyl jasmonate (MeJA) treatment was done 48 hours before harvest. nd, not determined.