Figure 8From: Alternative splicing and nonsense-mediated decay of circadian clock genes under environmental stress conditions in ArabidopsisEffects of heat on the alternative splicing of the clock genes. Ten-day-old Col-0 plants grown on MS-agar plates under LDs were transferred to 37°C under continuous light conditions. Whole plant materials were harvested at the indicated ZT points. The levels of the RNA splice variants of CCA1 (A), PRR7 (B), PRR9 (C), TOC1 (D), ELF3 (E), and ZTL (F) genes were determined by qRT-PCR. Biological triplicates were averaged. Bars indicate the standard error of the mean. Note that the expression data at 23°C are identical to those in Figure 7.Back to article page