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Figure 11 | BMC Plant Biology

Figure 11

From: Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1

Figure 11

Expression of stress-responsive genes in wild-type and transgenic plants subjected to salt stress. Relative expression levels of stress-responsive genes were determined by qRT-PCR using cDNA synthesized from total RNAs isolated from the leaves of 2-week-old Arabidopsis subjected to high-salt stress (150 mM NaCl) for 24 h. A, RD29A; B, RAB18; C, RD22; D, ERD15; E, KIN1. There were four treatments: WT CK represents wild-type plants grown under non-stressed conditions; 35S::CaMBF1 CK represents transgenic plants grown under non-stressed conditions; 35S::CaMBF1 NaCl represents transgenic plants subjected to salt stress; WT NaCl represents wild-type plants subjected to salt stress. Arabidopsis elF4A gene (At3g13920) was used as an internal control for normalization of different cDNA samples. The expression levels of stress-responsive genes in wild-type plants at 0 h were used as control (quantities of calibrator) and were assumed as 1. Three biological triplicates were averaged and Bars indicate standard error of the mean.

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