Figure 3

Analysis of CaMBF1 expression profiles in pepper seedlings in response to different stress treatments. The pepper seedlings were sprayed with 5 mM SA solution (A); the pepper seedlings were exposed to salt stress (300 mM NaCl) (B), osmotic stress (300 mM mannitol) (C) and heavy metal (300 μM Hg) (D) for the indicated times (0, 1, 3, 6, 12 and 24 h). Pepper UBI-3 gene (GenBank No. AY486137.1) was used as an internal control for normalization of different cDNA samples. The expression level of CaMBF1 at 0 h was used as control (quantities of calibrator) and was assumed as 1. Error bars represent standard error of means based on three independent reactions.