Figure 5

Transcriptome analysis reveals a global upregulation of mitochondrial and stress-responsive genes in transgenic P07 compared with WT. (A) Scatterplot of log₂ RPKM showing nuclear- (Red circle symbol) and mitochondrial (Blue circle symbol)-encoded mitochondrial proteins that were upregulated in the P07 AtUCP1 transgenic line compared with WT. The green dots represent the total differentially expressed genes. (B) Examples of genes associated with nuclear and mitochondrial-encoded mitochondrial proteins that were upregulated in the P07 transgenic line compared with WT. (C) Scatterplot of log₂ RPKM showing stress-responsive (Red circle symbol) genes that were upregulated in AtUCP1 transgenic lines compared with WT. The green dots represent the total differentially expressed genes. (D) Examples of genes associated with abiotic stress responses that were upregulated in the P07 transgenic line compared with WT. Normalization was performed by applying log₂ to the RPKM values of WT and the P07 transgenic line. All the represented transcripts showed significantly different expression between the genotypes (p < 0.05). The RPKM means were calculated using four biological replicates (n = 4) of L3 leaves from each genotype.