Transcriptional analysis of TaHKT2;1 gene family in T. aestivum L. cv. Chinese Spring. Tissue samples of each genotype were measured for ion concentration in triplicate and each genotype was replicated four times for each treatment. (A) Tissue Na+ concentrations in treatments with no added NaCl (dark grey) and 200 mM NaCl (light grey). (B) Nullisomic-tetrasomic analysis of gene specific primers used for transcript analysis. (C) RT-PCR and detection of FL- cDNA in roots, sheaths and leaf blades.