Characterization of the multigene family TaHKT 2;1 in bread wheat and the role of gene members in plant Na+ and K+status

Table 4 Gene specific PCR primers targeting INDELs and SNPs to amplify individual members of the TaHKT2;1 gene family

Gene	Primer	Primer location (bp)	Primer sequence 5′-3′	PCR and the annealing temperature (°C)
TaHKT2;1 7AL-2 and TaHKT2;1 7AL-3	5′A2/3 F	−56	GAGACCTATCTTGACACGCAT	Touch down PCR at 55-50
TaHKT2;1 7AL-2 and TaHKT2;1 7AL-3	5′A2/3 R	+489	GACTCATCACTTTGTGCTGC	Touch down PCR at 55-50
TaHKT2;1 7AL-1	A1 F	+1325	GCACCACCCAGTGATGAC	Touch down PCR at 55-50
TaHKT2;1 7AL-1	A1 R	+1894	CCAGAAAAGCTGTATCGCA	Touch down PCR at 55-50
TaHKT2;1 7BL-1 and TaHKT2;1 7BL-2	B1/2 F	+1353	GAGAACACGAAAGGGAGAGT	Touch down PCR at 55-50
TaHKT2;1 7BL-1 and TaHKT2;1 7BL-2	B1/2 R	+1933	TCCATAGAGCATGACCGAT	Touch down PCR at 55-50
TaHKT2;1 7DL-1	D F	+1357	CGAGCACAAAAGGCAAGA	Touch down PCR at 55-50
TaHKT2;1 7DL-1	D R	+1955	TGGACACCGCAAACTCT	Touch down PCR at 55-50
TaHKT2;1 7AL-1	FL:A1 F	−29	CATTTGTTTCTCCCAGTCG	Standard PCR at 53
TaHKT2;1 7AL-1	FL:A1 R	+2180	CCACACGTTGATAGATAATGTC	Standard PCR at 53
TaHKT2;1 7BL-1 and TaHKT2;1 7BL-2	FL:B1/2 F	−19	CACACTCATACATAGCACCAT	Standard PCR at 53
TaHKT2;1 7BL-1 and TaHKT2;1 7BL-2	FL:B1/2 R	+2251	TTCTCTTTCGCTACGATTGT	Standard PCR at 53
TaHKT2;1 7DL-1	FL:D F	−23	GTTTCTCACTCATATATAGGACCA	Standard PCR at 56
TaHKT2;1 7DL-1	FL:D R	+2039	GTACGCAGCAGTTATACCAG	Standard PCR at 56

ISSN: 1471-2229