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Figure 9 | BMC Plant Biology

Figure 9

From: The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora perniciosa

Figure 9

Deoxyribonuclease activity of recombinant TcPR-4b. A and B. DNase activity of the TcPR-4b tested against 1 μg of plasmidial DNA (pGEM-T® Easy Vector; Promega). C. DNase activity of the TcPR-4b tested against 1 μg of genomic DNA from Nicotiana tabacum. The incubation with TcPR-4b was made overnigth at 25°C. The boiling conditions were 10 min at 95°C. The following concentrations were used: 10 mM of MgCl2, 10 mM of EDTA; 1 mM CaCl2. gDNA: genomic DNA; pDNA: plasmidial DNA.

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BMC Plant Biology

ISSN: 1471-2229

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