Figure 2From: An Arabidopsis mutant impaired in intracellular calcium elevation is sensitive to biotic and abiotic stressMutants which do not respond to A. brassicae CWE. 18-day old M2 seedlings from the individual M1 plants were used for the mutant screening. About 70% of the roots from the individual M2 seedlings was dissected and incubated in 7.5 μM coelenterazine overnight and challenged with the CWE. cycam1, cycam2 and cycam3 did not respond to the CWE.Back to article page