Figure 1

Constructs used for transgene introduction in Arabidopsis. (a) Genes stacked in COX + and COX++ lines by crossing: HMGR1S, short (cytosolic) isoform of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1; FPS1L, farnesyl diphosphate synthase 1 long isoform; FaNES1, nerolidol synthase 1 from Fragaria x ananassa; COX, mitochondrial signal peptide; P35S, CaMV 35S promoter; Tnos, terminator of the Agrobacterium tumefaciens nopaline synthase. (b) Average signal intensities of the overexpressed (HMGR1S and FPS1L) and housekeeping (GADPH and ACT2) genes in Col-3 (WT), COX + and COX++ lines. Bars indicate the 95% confidence interval.