Table 1 Primer sequences used for CsCCD7 and CsCCD8 genes cloning and analysis
|  | Sequences | Primers (5′-3′) |
|---|---|---|
Degenerate primers | CCD8-F0 | GTSGTGAGRATGGAASCHGG |
CCD8-F1 | GNCAYYTNTTYGYGGNTAYGC | |
CCD8-F2 | CNCCNYTNTAYAARTTYARTGGCA | |
CCD8-F3 | ATMCCAYTKGAYGGRAGC | |
CCD8-R0 | CCATCATCYTCWTSGGTGC | |
CCD8-R1 | CCAYTRAAYTTRTANARNGGNGT | |
CCD8-R2 | TNGTNARNGTRTTNGGRAARCART | |
CCD8-R3 | CCARCADCCRTGVARGCC | |
CCD7-F0 | GACGAYCAYGGYTCCACSGTSCAC | |
CCD7-F1 | CTCGACGGCCAYGGYTACCT | |
CCD7-R1 | CYCKRTGCGTRAACCRCCACGA | |
Genome walker | CCD8-F1 | GGTGTGGTTATSTCTGTGATAAGTG |
CCD8-F2 | CCAACTTCGAGGAGTTGCACGA | |
CCD8-R1 | ATGCTCATTCGGGTCGAGTGCT | |
CCD8-R2 | CTTTGTCGCTCCCATCCAATGGT | |
CCD8-R3 | TTGCATGGCCTCACAGCTCCA | |
CCD8-R4 | CAACTCTCCTTTGTCGCTCCCATC | |
CCD8-R5 | TGCCGTTGATGAAGTGGAAGGTCAG | |
CCD8-R6 | TGTTGGCGTTGTCGCTGAGTGAC | |
CCD8-R7 | AGAAGTTGTCGGTCTTCGGGACT | |
CCD8-R8 | TACATGCTAGCCACAAGGGCTCCA | |
CCD8-R9 | TGGTCTGGCGTTCGGTAGGTGCT | |
CCD7-F3 | TCTCGGTGAACCCAAGCCAGCA | |
CCD7-F4 | GCAGACATCACCCATATATCTGCT | |
CCD7-F5 | TCTCGGTGAACCCAAGCCAGCA | |
CCD7-R1 | CAAGACAAAAAGGAATGGCA | |
CCD7-R2 | CAGTCGTGGATCATCAACTGGTCTG | |
CCD7-R3 | TGTCTCCATCTGTTGTCTGGTTG | |
CCD7-R4 | ATGATGGAGCTTATGTTGGGTCA | |
cDNA isolation | CCD7-F | ATGCACTCCATTTCTCACCGC |
CCD7-R | TAGTGATTTCTGCGAGACCA | |
CCD8a-F | ATGGCAGACGTAGGGATACTGA | |
CCD8b-F | CATGCAAACAAACTTAATAGCT | |
CCD8-R | CTTGTCCTCTATTAAATTACTTC | |
QRT-PCR expression analysis | CCD7-F | ACCTCCCCGTCATCCAAT |
CCD7-R | ATGACGGTTTCGGTCTCG | |
CCD8-F | GAAGGGGTCGATCGAGGT | |
| Â | CCD8-R | CGTCGCCGTACTCGAACT |