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Figure 2 | BMC Plant Biology

Figure 2

From: Functional analysis of COP1 and SPA orthologs from Physcomitrella and rice during photomorphogenesis of transgenic Arabidopsis reveals distinct evolutionary conservation

Figure 2

Complementation analysis of Arabidopsis cop1-4 hypomorphic mutants carrying the rice, Physcomitrella or Arabidopsis COP1 transgene. A, B. Visual phenotype of cop1-4 mutant Arabidopsis seedlings that are homozygous for the transgenes AtCOP1 (Arabidopsis COP1), OsCOP1 (rice COP1) or PpCOP1a (Physcomitrella COP1). Seedlings were grown in darkness (A) or red light (B, 5 μmol m-2 s-1) for four days. Three independent transgenic lines and, as controls, wildtype Col (WT) and a cop1-4 mutant are shown. C. Visual phenotype of cop1-4 mutant Arabidopsis plants. Genotypes were as in B. Plants were grown in short day for four weeks. D, E. Scatter plot representing leaf length (D) and flowering time (E) of 25–27 individual, i.e. independent T1 primary transformants and 15 individual wild-type and cop1-4 mutant control plants. Plants were grown in short day.

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