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Figure 3 | BMC Plant Biology

Figure 3

From: Functional analysis of COP1 and SPA orthologs from Physcomitrella and rice during photomorphogenesis of transgenic Arabidopsis reveals distinct evolutionary conservation

Figure 3

Complementation analysis of Arabidopsis cop1-5 null mutants carrying the rice, Physcomitrella or Arabidopsis COP1 transgene. A. Visual phenotype of cop1-5 null mutant Arabidopsis seedlings that are homozygous for the transgenes AtCOP1, OsCOP1 or PpCOP1a. Seedlings were grown in darkness for four days. WT (Ws) and three independent transgenic lines are shown. cop1-5 mutant seeds failed to germinate due to the seedling-lethal phenotype and are therefore not shown. B. Hypocotyl elongation response of transgenic cop1-5 mutant seedlings to blue light. Genotypes were as in A. Error bars show the standard error of the mean (SEM). C. Visual phenotype of transgenic cop1-5 mutant Arabidopsis plants. Genotypes were as in A; one representative transgenic line is shown for each transgene. Plants were grown in short day for three weeks. D, E. Leaf size (D) and flowering time (E) of homozygous transgenic cop1-5 lines. Genotypes were as in A. Two to three independent transgenic lines are shown for each construct. Wild type (Ws) serves as a control. The cop1-5 mutant is seedling-lethal and therefore not shown. Rather, cop1-4 and WT (Col) are shown as controls to allow evaluation of growth conditions. Error bars show the SEM, n = 12.

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