Figure 4

SPA1 orthologs from rice and Physcomitrella do not complement seedling nor adult phenotypes of Arabidopsis spa1 spa3 spa4 mutants in the light. A. Visual phenotype of spa1 spa3 spa4 mutant Arabidopsis seedlings that carry constructs with the coding sequence of Arabidopsis AtSPA1 or AtSPA4, rice OsSPA1 or Physcomitrella PpSPAb driven by the Arabidopsis AtSPA1 or AtSPA4 promoters (pAtSPA1, pAtSPA4). Seedlings were grown in weak red light (0.1 μmol m^-2 s^-1) for four days. B. Visual phenotype of plants grown in short day for four weeks. Genotypes are as in A. C, D. Scatter plot showing leaf length (C) and flowering time (D) of 18–24 individual, i.e. independent T1 primary transformants carrying the transgenes described in (A) and 15 individual wild-type and spa1 spa3 spa4 mutant control plants. Plants were grown in short day.