Figure 5From: Functional analysis of COP1 and SPA orthologs from Physcomitrella and rice during photomorphogenesis of transgenic Arabidopsis reveals distinct evolutionary conservationTranscript levels of the transgenes in transgenic spa1 spa3 spa4 mutant lines. AtSPA1, OsSPA1, PpSPAb and AtSPA4 transcript levels in transgenic seedlings carrying the indicated constructs. Transcript levels were analyzed by semi-quantitative RT-PCR using primers specific for the respective transgene-encoded transcript. Seedlings used for RNA isolation were grown in weak red light (0.1 μmol m-2 s-1) for four days. Primers amplifying the ACT2 transcript were used as a control.Back to article page