Figure 3

Phylogenetic tree based on amino acid sequences of MIKC-type MADS-box genes. 60 MIKC-type MADS-box genes were used: 16 from Bambusa edulis, 16 from rice (Oryza sativa), 10 from maize (Zea mays), and 18 from wheat (Triticum aestivum L.). Deduced full-length amino acid sequences were used for the alignments. The phylogenetic tree was constructed by the neighbor-joining method and evaluated by bootstrap analysis (MEGA version 4.0). Numbers on major branches indicate bootstrap percentage for 1,000 replicates. Six Arabidopsis sequences of the FLC subfamily were used as outgroups. Proteins from B. edulis were highlighted with red boxes. The three grass clades of FUL1, FUL2, and FUL3 within the AP1 subfamily and the two major clades of the SEP subfamily are labeled on the right. The five grass clades within the SEP subfamily are indicated by numbers showing their respective name according to previous studies [41], namely 1: LHS1/OsMADS1, 2: OsMADS5, 3: OsMADS34, 4: OsMADS7/45, 5: OsMADS8/24. Subfamilies of the plant MIKC-type genes and the functional classification according to the A/B/C/D/E classes are indicated at the right margin.