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Table 1 List of sequences and primers designed to perform SemiQ RT-PCR experiments

From: Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis(L.) Osbeck

GST class

Sequence ID

Primer features

  

Forward (5′-3′)

Reverse (5′-3′)

Annealing temperature (°C)

Expected amplicon (nt)

Tau

CITSI24:CX077363

TCGGCATGGAAGAAGTGAAGCTACTGGGA

ACCGTTCAAAAGATAGTGTCTATGAAGTA

65

784

CITSI47:3

TCGCCAAGCCCATTTGTGATGAGGGCA

ACGAGACAGGCTGCTGCCTAGTCCGA

66

866

CITSI57:1

AGTAAGCTTCTGTAATAATGGCGGACGA

ACAATACCCTAAGATAACAGTCGGGGACA

64

879

CITSI43:1

TCTGTCACAATGGCGGACGAAGTGGT

AGCAGGCAGCACGATTGCGCTGCT

68

732

CITSI16:1

TCACTCGCCCTTAATTCTCAGTAAGGT

AGATTGACGCCACATAATATTCCCA

60

966

CITSI53:1

TGCTGGGTTACTGGGCAAGCCCCT

ACCTTCATGCATGGGCAACCGCTGA

66

686

CITSI41:1

GTTTACAGGGTGATTTGGGCTCTGA

ACCACTATGCTAGTCCCCCGAACT

63

757

CITSI25:1

AAGCTCTATGGCACATGGGTAAGCCCTT

ACACAGAGAGAGAGCTAACCCAATCA

65

730

CITSI51:1

TGGCCAAGCCCGTTTGTGTTTAGGGT

AGACTTTCCACACAACATCACACTAC

63

756

CITSI36:1

GCGAAAATAATATGGCCAAAGAAGTGACGCT

GTCATTACAACACACCACAACACCACCT

64

765

CITSI04:1

AGACGTGGTCAAGCCCCTTTGGT

TGGAATGGGAAAAGGGCAAAAGGA

62

889

CITSI44:1

GCAGAAGATTATGGCAACAAAAGTG

GAGCGTACAGAAAGGAGACACGTGCA

62

764

Phi

CITSI52:1

AAGCTCTTCGAATCAAGGGCAATCACCCAA

TTTTGATAAACCCATTGGGACAGTCGT

65

800

CITSI38:CK934228

ATACGAATCTCGAGCTATCATAAGGTACTA (a)

TTTTCTCCCAAGGCCCCAAGCATTT

64

636

CITSI23:1

ATACGAATCTCGAGCTATCATAAGGTACTA (a)

TTTTGTCCTCCAAGGCCCCAAGCAT

64

638

CITSI34:CK939385

ATACGAATCTCGAGCTATCATAAGGTACTA (a)

TTTTGTCCTCCAAGGCCCCCAGCAT

65

678

CITSI33:1

TTTATACGAGTCGCGAGCTATCATGAGGT

TTTTAAAGCTCCAACTCCAAACAT

60

658

CITSI05:DY257328

ATTTTATACGAGTCGCGAGCTATCATGAGG

ACCCCTTATCCAAGGAACATTTCCCA

64

565

CITSI11:1

TGGGGATTTTACTCTATACGAATCGCGA

ATGGCGACAACAAGAAATCGCCGCA

63

640

DQ198153 (CITSI02:1 + CITSI00:1)

GCTTTTTGAGTCGAGGGCAATCATAAGG

GATAACAGTAATGACAGCCAGCCGAA

64

549

CITSI29:1

AGGATGAGAAAATCTCCCTCTTAGAGTCT

TGGGAAATTATTAGACCATGCCA

63

748

CITSI18:1

ATTTGAATCTAGAGCAATGACAGCATACGT

CATCAATGTAAAATCATCACGCAACCA

63

599

Lambda

CITSI46:1

CCTCCAAGATAGGCCCGCTTGGTAC

TCCAGCAACGTACACAAGCTCACATCGGCA

66

672

Zeta

CITSI21:1

ATGCTGAAACTGTATTCATACTGGAGGAGT

TGCTGCTTATTGAGGGTCAACAAAGGCTG

64

880

Mapeg

CITSI20:1

CGACTCGACTATGGCGGATGCAAC

CTATGAGCTTATGCTTGCGCCATGCAGC

66

638

  1. GST classes, oligonucleotides sequences and the expected size of amplicons (on the basis of correct maturation) are highlighted. Uppercase letters indicated the use of the same forward primer.