Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis(L.) Osbeck

Table 2 Summary of tissue/organ expression profiling by SemiQ RT-PCR

GST Class	Sequence ID	Cultivar	SemiQ RT-PCR
			Albedo	Flavedo	Flesh	Young leaf	Adult leaf	Ovary
Tau	CITSI24:CX077363	C	+	+	++	+++^a	++++^a*	++++^a*
	CITSI24:CX077363	M	+	+	+++	++^a	++^a*	++^a*
	CITSI47:3	C	++	++	++	++^a	++	+++^b*
	CITSI47:3	M	+^a	++	+	++^a	++	-*
	CITSI57:1	C	++^a	+^a	+++^a	++^a	++++^a	++++^a*
	CITSI57:1	M	++^a	++^a	++++^a	++^a	+++^a	-*
	CITSI43:1	C	++	++	+++	+++	+++	++++^a*
	CITSI43:1	M	++	++	+++	+++	+++	++*
	CITSI16:1	C	+++	-	+++	+++	+++	-
	CITSI16:1	M	+++	-	++	+++	+++	-
	CITSI53:1	C	+++	+++	+++	+++	+++	+++^a
	CITSI53:1	M	+++	+++	+++	+++	+++	+++
	CITSI41:1	C	+	++	+++	++^a	+++^a	+++^a
	CITSI41:1	M	+	++	+++	++^a	++	++
	CITSI25:1	C	++	+	+	+++	++	+
	CITSI25:1	M	+	+	++	+++	++	++
	CITSI51:1	C	++	++	++	-	++	++^b*
	CITSI51:1	M	+	++	++	-	++	-*
	CITSI04:1	C	-	-	-	-	++	-
	CITSI04:1	M	-	-	-	-	+	-
	CITSI44:1	C	++	++	+++	++	+++	++^b*
	CITSI44:1	M	+++	+++	++	+++	+++	-*
	CITSI36:1	C	++	++	+++	++++	+++	+++^a
	CITSI36:1	M	+++	++	+++	++++	+++	++
Phi	CITSI52:1	C	+	+	+	++	++	+
	CITSI52:1	M	-	+	+	++	++	+
	CITSI38:CK934228	C	-	+	++*	++	+++	+
	CITSI38:CK934228	M	-	+	-*	++	++	-
	CITSI23:1	C	-	+	++	++	++	+
	CITSI23:1	M	-	+	+	++	++	-
	CITSI34:CK939385	C	-	+	-	++	+++*	+
	CITSI34:CK939385	M	-	+	-	+	+*	-
	CITSI33:1	C	+++	++	+++	+++	++	+++
	CITSI33:1	M	+++	++	++	+++	+++	++
	CITSI05:DY257328	C	++	++	++	++	++^a	++
	CITSI05:DY257328	M	++	++	++	++	++	+
	CITSI11:1	C	+	-	++	-	+	++
	CITSI11:1	M	+	+	++	+	+	+
	DQ198153	C	-	-	+*	+	+	-
	DQ198153	M	-	+	++++*	+	+	-
	CITSI29:1	C	-	-	+	++	+++	+
	CITSI29:1	M	-	-	+	++	++	+
	CITSI18:1	C	-	-	-	-	+	+
	CITSI18:1	M	-	-	-	-	-	-
Lambda	CITSI46:1	C	+++	+++	++++	++	++++	++++
Lambda	CITSI46:1	M	+++	+++	++++	+++	++++	+++
Zeta	CITSI21:1	C	++^a	+^a	+++^a	++^a	++^a	++^a
Zeta	CITSI21:1	M	++^a	++^a	+++^a	++^a	++^a	++^a
Mapeg	CITSI20:1	C	++	++	+*	++	+++	+++
		M	+++	++	+++*	+++	+++	++

‘C’ and ‘M’ indicate the Cadenera and Moro cultivars, respectively. Expression level is indicated as '–' (no expression) or from '+' (weak) to '++++' (strong) depending on the presence of the amplicon at the PCR cycle 20 (+), 25 (++), 30 (+++) and 35 (++++).
Uppercase letters indicate:
^athe presence of multiple-bands.
^bRT-PCR products with size greater than the expected on the basis of the primer design on the transcripts.
*differences in the expression levels of more than ten cycles between the two cultivars.

ISSN: 1471-2229