Lotus tenuis x L. corniculatus interspecific hybridization as a means to breed bloat-safe pastures and gain insight into the genetic control of proanthocyanidin biosynthesis in legumes

Table 1 PA content in leaves, stems, flowers and roots of parental and F1 hybrid plants

Sample		Soluble proanthocyanidins					Insoluble proanthocyanidins					Total proanthocyanidins
Sample		Mean		S.D.	Ranks		Mean		S.D.	Ranks		Mean		S.D.	Ranks
Leaves	Lc	10.84	±	1.51	65.5	e	12.3	±	4.14	63.5	c	25.85	±	4.85	65.5	d
	*LH1*	1.08	±	0.18	41.6	cd	4.71	±	0.98	37.3	b	5.79	±	0.94	37.8	bc
	*LH2*	0.58	±	0.30	25.7	bc	4.25	±	1.58	32.3	b	4.83	±	1.67	29.8	b
	*LH3*	0.50	±	0.12	24.0	b	3.97	±	1.92	29.3	b	4.47	±	1.91	27.8	b
	*LH4*	1.71	±	0.58	50.8	de	5.86	±	1.99	45.2	b	7.58	±	2.42	46.7	c
	Lt	0.06	±	0.02	6.0	a	0.93	±	0.34	6.0	a	0.99	±	0.34	6.0	a
Stems	Lc	0.37	±	0.16	52.5	d	2.49	±	1.15	46.9	c	2.82	±	1.13	48.5	c
	*LH1*	0.07	±	0.04	23.7	abc	1.16	±	0.70	24.6	b	1.23	±	0.73	24.3	b
	*LH2*	0.11	±	0.06	31.2	bc	1.47	±	0.41	36.5	bc	1.58	±	0.42	36.0	bc
	*LH3*	0.14	±	0.06	36.8	c	1.07	±	0.35	24.9	b	1.20	±	0.36	26.3	b
	*LH4*	0.05	±	0.04	17.1	ab	1.07	±	0.24	26.7	b	1.11	±	0.26	24.6	b
	Lt	0.03	±	0.01	11.1	a	0.40	±	0.04	6.5	a	0.43	±	0.04	6.3	a
Flowers	Lt	12.43	±	2.36		d	14.55	±	2.79		c	26.97	±	0.76		c
	*LH1*	9.14	±	1.84		bc	11.97	±	4.07		bc	21.11	±	5.87		bc
	*LH2*	9.62	±	0.14		cd	14.17	±	2.61		c	23.80	±	2.59		c
	*LH3*	6.19	±	1.57		ab	7.65	±	4.11		ab	13.84	±	2.94		a
	*LH4*	8.97	±	1.47		bc	6.78	±	1.96		ab	15.74	±	3.21		ab
	Lc	3.88	±	2.22		a	5.70	±	3.18		a	9.58	±	4.33		a
Roots	Lc	0.46	±	0.13		a	0.65	±	0.10		b	1.12	±	0.19		ab
	*LH1*	0.82	±	0.34		bc	0.72	±	0.11		bc	1.54	±	0.38		bc
	*LH2*	0.86	±	0.18		c	0.66	±	0.17		bc	1.52	±	0.34		bc
	*LH3*	0.47	±	0.18		a	0.36	±	0.14		a	0.83	±	0.30		a
	*LH4*	0.50	±	0.10		ab	0.36	±	0.14		a	0.86	±	0.21		a
	Lt	0.74	±	0.18		bc	0.96	±	0.10		c	1.70	±	0.26		c

Samples are as in Figure 2. Values are expressed as mg PA g-1 of dry weight ± standard deviation. Within each organs block means with a similar letter do not differ significantly (p < 0.05).

ISSN: 1471-2229