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Figure 3 | BMC Plant Biology

Figure 3

From: A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement

Figure 3

Cytological and molecular analysis of M. oryzae in rhizome tissues. (a) Rhizome tissue sections were stained by Toluidine blue and visualized by light microscopy. Brown deposition/Rice blast lesions in individual epidermal cells were observed (arrow) but did not show spreading of the lesions on the rhizome tissue surface. Bar = 200 μm. (b) Fungal conidia were observed on the surface of rhizome (arrow) by scanning electron micrograph (SEM). Bar = 50 μm. (c) No fungal conidia or infection hyphae on red rice leaf tissues were detected. Bar = 100 μm. (d) The red rice plants grew in the greenhouse without any typical symptoms of rice blast/M. oryzae. (e) Fungal hyphae (arrow) spread on the Petri dish from the rhizome tissue with three days of culture on potao dextrose agar (PDA). (f) Fungal hyphae and spores (arrow) grew on the Petri dish from the rhizome tissue with eight days of culture on PDA. (g) An M. oryzae-specific gene was detected by RT-PCR, showing amplification of the predicted specific gene sequence 156 bp fragment in hyphyae/mycelia (lane 1) and rhizome (lane 2), but not in root (lane 3) and leaf (lane 4). L: DNA ladder.

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