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Figure 1 | BMC Plant Biology

Figure 1

From: ENHANCING CTR1-10 ETHYLENE RESPONSE2 is a novel allele involved in CONSTITUTIVE TRIPLE-RESPONSE1-mediated ethylene receptor signaling in Arabidopsis

Figure 1

Gene structure and ethylene-dose response assay of the mutant constitutive triple response 1–10 ( ctr1-10 ). (A) ctr1-10 gene structure and cDNA sequence flanking the T-DNA. The T-DNA is in gray and CTR1 coding region white. (B) The T-DNA sequence is transcribed as part of the ctr1-10 5′-untranslated region (5′-UTR). Arrows indicate the primer orientation and corresponding cDNA sequences for RT-PCR. Capital letters are the T-DNA sequence and lowercase letters are the CTR1 cDNA sequence. T-DNA is in gray and the 5′-UTR white. RT-PCR analysis of gene expression. Ethylene dose–response assay for hypocotyl measurement (C) and relative hypocotyl length (D) of etiolated wild-type (Col-0) and ctr1-10 seedlings. The dotted line in (D) indicates 50% growth inhibition. Data are mean ± SD (n ≥ 20).

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BMC Plant Biology

ISSN: 1471-2229

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