Figure 6
From: Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2
Nucleotide and amino acid polymorphisms of the DFR2 gene in flower color variants of soybean. (A) Amino acid sequence of Bay and 222-A-3. Amino acids polymorphic in 222-A-3 are shown in bold. NADPH binding domain is underlined. The polymorphic amino acid in E30-D-1 is shown in red font. End of the fourth exon is indicted by an arrow. (B) Alignment of partial cDNA and amino acid sequences from Bay and 222-A-3. Polymorphic nucleotides are shown in red font. The polymorphic amino acids in 222-A-3 are shown in bold. (C) Alignment of partial cDNA and amino acid sequences from Bay and E30-D-1. Polymorphic nucleotides and amino acids are shown in red font. Restriction site used for CAPS analysis is underlined. (D) Alignment of partial nucleotide and amino acid sequences around the end of the fourth exon of Clark and the corresponding region of Clark-w4. Polymorphic nucleotides are shown in red font. Nucleotides common in the 5′ splice site are underlined. Amino acids polymorphic in Clark-w4 are shown in bold.