Figure 7
From: Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2
Results of CAPS analysis of DFR2 gene in soybean. (Upper panel) Results of CAPS analysis for flower color variants. PCR products amplified with CAPS primers were digested by BsrGI and the digests were separated on an 8% polyacrylamide gel. ф, molecular marker фx174/HaeIII; B, Bay; C, Clark; C4, Clark-w4; 22, 222-A3; E3, E30-D-1. (Lower panel) Results of CAPS analysis in an F2 population derived from a cross between E30-D-1 and Clark-w4. ф, фx174/HaeIII; C4, Clark-w4; E3, E30-D-1; H, F2 plants segregating for flower color; L, F2 plants fixed for light purple flower; N, F2 plants fixed for near white flower. The migration of size markers is shown to the left of the gel.