Reduced polyphenol oxidase gene expression and enzymatic browning in potato (Solanum tuberosum L.) with artificial microRNAs

Table 2 List of the amiRNA constructs, amiRNA names, amiRNA target sequences and target genes

Construct	amiRNA name	amiRNA sequence (5′ → 3′)	Target sequence* (5′ → 3′)	Gene name (GenBank accession ID)
pPZamiRPPO1	amiRPPO1	UUGGUGACUGGUGCAAUUGAC	GUCAAUUGCACCAGUCACCAA	StuPPO1/POTP1/P2 (XM_006355177/M95196/M95197)
pPZamiRPPO2	amiRPPPO2	UUGCUAGCUGGCGGAAGUGAA	UUCACUUCCGCCAGCUAGCAA	StuPPO2/POT32 (U22921)
pPZamiRPPO3	amiRPPO3	UUGUUCACUGGGGGGAGUGUA	UACACUCCCCCCAGUGAACAA	POT33 (U22922)
			UUCACUCCCCCCAGUGAACGA	StuPPO3 (XM_006365320)
pPZamiRPPO23	amiRPPO23	UCAUCAACUGGAGUUGAGUUG	CAACUCAACUCCAGUUGAUGA	StuPPO2/POT32
			CAACUCAACUCCAGUUGAUGA	StuPPO3/POT33
pPZamiRPPO234	amiRPPO234	UAGAACUCGGAGUUCAACCAA	UUGGUUGAACUCCGAGUUCUA
			UUGGUUGAACUCCGAGUUCUU	StuPPO2/POT32
			UUGGUUGAACUCCGAGUUCUU	StuPPO3 (XM_006365320)
			UUGGUUGAACUCUGAGUUCUU	POT33 (U22922)
			UUGGUUGAACUCCGAGUUCUU	StuPPO4/POT72 (U22923)
pPZamiRPPO234A	amiRPPO234A	AAGAACUCGGAGUUCAACCAA	UUGGUUGAACUCCGAGUUCUU	StuPPO2/POT32
			UUGGUUGAACUCUGAGUUCUU	StuPPO3/POT33
			UUGGUUGAACUCCGAGUUCUU	StuPPO4/POT72
pPZamiRPPO1234	amiRPPO1234	UCAAGCUCAUUCGCAUUCACA	UGUGAAUGCGAAUGAGCUUGA
			UGUGAAUGCGGAUGAGCUUGA	StuPPO1/POTP1/P2
			UGUGAAUGCAAAUGAGCUUGA	StuPPO2 (XM_006365321)
			UGUGAAUGCGAAUGAGCUUGA	POT32 (U22921)
			UGUGAAUGCGAAUGAGCUUGA	StuPPO3/POT33
			UGUGAAUGCGAAUGAGCUUGA	StuPPO4/POT72

Note: * The non-identical nucleotides, compared to the target sequence, are marked in bold. The homologous sequences of the predicated PPO-like gene models (StuPPO1 to StuPPO9) to all amiRNA target sequences are aligned in the Additional file 1: Part D.

ISSN: 1471-2229