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Table 1 Comparison between the expected and observed N-terminal amino acid sequences of the eight transgenic proteins

From: An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm

Genotype

Spot n.

Expected N-terminal sequence

Observed N-terminal sequence

42K-N23T

1

METSHIPSLEKPLQQ-

METSHIP-

B1133-WT

3

METSHIFGLERPWQQ-

METSHIFGLERPWQQ-

 

4

METSHIFGLERPWQQ-

METSHIFGLERPWQ-

B1133-T23N

5

  SCIPGLERPWQQQPL-

  SCIPGLERP-

 

6

  SCIPGLERPWQQQPL-

  SCIPGLERP-

 

7

  SCIPGLERPWQQQPL-

  SCIPGLERPW-

 

8

  SCIPGLERPWQQQPL-

  SCIPGLERP-

  1. In the “Genotype” column the three transgenic genotypes here analysed are reported. The “Expected N-terminal sequence” column reports the deduced N-terminal amino acid sequences on the basis of transgene nucleotide sequences, whereas the “Observed N-terminal sequence” corresponds to those obtained after Edman degradation, relatively to each of the eight protein spots indicated in the second column.
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BMC Plant Biology

ISSN: 1471-2229

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