Figure 2

Arabidopsis TEV-susceptibility protein AteIFiso4E does not interact with TEV VPg in yeast two-hybrid assays. Yeast transformed with both bait (fused to the Gal4 binding domain, BD) and prey (fused to the Gal4 activation domain, AD) vectors were spotted on selective dropout medium without leucine and tryptophan (SD-LW) as a control and on selective dropout without leucine, tryptophan and histidine (SD-LWH) to check for interaction between both partners. In each case, a control with an empty vector (-) was included to confirm there was no self-activation. All combinations are shown in triplicate.