Figure 3From: Specific requirement for translation initiation factor 4E or its isoform drives plant host susceptibility to Tobacco etch virusThe rtm1 eifiso4e double mutant is resistant to TEV HAT and TEV CAA10 does not infect Arabidopsis thaliana L er or Col accessions. One-month-old Arabidopsis were manually inoculated with TEV HAT or CAA10 strains and assayed at 24 dpi. Wild type accessions Columbia (eIFiso4E/eIFiso4E; RTM1/RTM1) and Landsberg erecta (eIFiso4E/eIFiso4E; rtm1/rtm1) were compared to the double mutant eifiso4e/eifiso4e; rtm1/rtm1. Nicotiana tabacum cv Xanthi non-inoculated (mock) or inoculated (Inoc) plants were included as controls. A, Plants were assayed for viral coat protein accumulation by ELISA at 24 dpi. Mean values for 6 independent plants per genotype are shown and error bars represent standard errors. The horizontal black line is the susceptibility threshold. B, RT-PCR expression of the TEV VPg gene in systemic leaf tissues. APT1 is amplified as a constitutive control in Arabidopsis plants.Back to article page