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Figure 4 | BMC Plant Biology

Figure 4

From: APUM5, encoding a Pumilio RNA binding protein, negatively regulates abiotic stress responsive gene expression

Figure 4

Effect of salt and dehydration stress on growth of wild-type and APUM5 transgenic seedlings. (A) The germination ability of wild-type, 35S-APUM5, and APUM5-RNAi plants was measured on 1/2 MS medium containing 150 mM NaCl, and germination was scored at the indicated days. (B) The germination test of wild-type, 35S-APUM5, and APUM5-RNAi transgenic plants upon 1/2 MS medium containing 400 mM mannitol. These experiments were performed for three independent sets (60–70 seeds per experiment). (C) Normal root growth of Col-0 and APUM5 transgenic plants. (D) The effect of 150 mM NaCl on primary root elongation. (E) The effect of 400 mM mannitol on primary root elongation. Seeds were germinated for 3 days on 1/2 MS medium and the seedlings were transferred (n = 30, triplicates) to 1/2 MS containing 150 mM NaCl or 400 mM mannitol. Primary root length was measured 7 days after transfer. Error bars indicate standard deviations (n = 3). (Student’s t-test; *P < 0.01).

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