Figure 5
Size separation of debranched endosperm starch and purified amylopectin by gel-filtration. Size separation of debranched endosperm starch and purified amylopectin from double mutant (E and I), parental mutant (C, D, G and H), and wild-type lines (A, B and F) by gel filtration chromatography through three Toyopearl HW55S-HW50S columns. Graphs show elution profiles of isoamylase-debranched starch (blue lines) and purified amylopectin (red lines). Different X-axis scales reflect different flow rates among experiments. Fractions (Fr. I, II, and III) were divided at troughs of carbohydrate content curve, as detected by refractive index detectors (left Y-axis). Grey line in wild type (Nipponbare) indicates λmax values of starch-iodine complexes (right Y-axis). Figures show one typical dataset (one of at least three replicates prepared from starch and purified amylopectin).