Figure 2

Confocal images of Pinot Noir berries (100- μm sections) sampled at the green hard, green soft and pale red stages, stained for H ₂ O ₂ and¹O ₂ . The sections were incubated with either 30 μM DCFDA or 30 μM SOSG (ROS sensors). Chlorophyll fluorescence has been recorded (Chl) to localize chloroplasts inside the cells. Merge is the computed overlay of the two fluorescence images and the bright field. Reference bars are 75 μm for H₂O₂ imaging and 25 μm for ¹O₂. Skin and pulp are indicated in the merge pictures with a “s” and “p”, respectively. For ¹O₂ imaging, only skin is visualized, at a higher magnification.