Figure 9
PnLOXA localization demonstrated by the expression of YFP fusion constructs in grapevine leaves. Leaves were infiltrated with Agrobacterium tumefaciens carrying the pGreen[PnLOXAtransitpeptide1-47-YFP] and pGreen[PnLOXAtransitpeptidePLAT1-220-YFP] constructs. Chlorophyll (Chl) and YFP fluorescence were recorded using Leica SP II confocal microscope. Merge is the computed overlay of the two fluorescence images. Reference bar is 10 μm.