Figure 1From: Galactosyltransferases from Arabidopsis thaliana in the biosynthesis of type II arabinogalactan: molecular interaction enhances enzyme activityIdentification of donor substrate for recombinant AtGALT29A. Affinity purified AtGALT29A (■) or P19 (□) was incubated with A: NDP-[14C]-sugars: UDP-[14C]-Xyl, UDP-[14C]-Glc, GDP-[14C]-Man and GDP-[14C]-Fuc (as MixI), and UDP-[14C]-GlcNAc, UDP-[14C]-GlcA and UDP-[14C]-Gal (as MixII); B: or individual NDP-[14C]-sugars from MixII using GAGP8 as acceptor substrate. Error bars showed standard deviations from n = 4. The result indicates that UDP-[14C]-Gal serves substrate for AtGALT29A.Back to article page