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Figure 3 | BMC Plant Biology

Figure 3

From: Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane

Figure 3

Co-bombardment and qRT-PCR assays reveal the synthetic luc * and Ren luc * transgenes produce increased levels of steady state mRNA relative to the native luciferase transgenes. A. qRT-PCR on equimolar serial dilutions of pUbi-luc (orange lines) and pUbi-luc* (blue lines) plasmid template using the corresponding gene-specific primer pair (Additional file 4 Figure S4A&B). B. Amplification from cDNA synthesised from suspension cells co-bombarded with equimolar amounts of pUbi-luc and pUbi-luc* plasmid. C. luc* transcript levels were on average 8.9-fold higher than luc transcript levels in the co-bombarded suspension cells cDNA. Error bars represent the standard error of the mean across three independent co-bombardments. D. qRT-PCR on equimolar serial dilutions of pUbi-Renluc (green lines) and pUbi-Renluc* green lines) plasmid using the corresponding gene specific primer pair (Additional file 4: Figure S4A&B). E. Amplification from cDNA synthesised from suspension cells co-bombarded with equimolar amounts of pUbi-Renluc and pUbi-Renluc* plasmid. F. Synthetic Renluc* transcript levels were on average 6.5-fold higher than the native Renluc transcript levels in the co-bombarded suspension cells cDNA. Error bars represent the standard error of the mean across three independent co-bombardments.

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