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Figure 3 | BMC Plant Biology

Figure 3

From: Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots

Figure 3

Expression of transcripts from each gene in transformed roots. RNA was converted to cDNA and used as template for PCR amplification of a fragment of each gene. Agarose gel containing amplicons representing a portion of AtNPR1, AtTGA2, AtPR-5, AtACBP3, AtACD2, AtCM-3, AtMC2, AtCDR1, AtDND1, respectively. Molecular weight markers (MWt) are shown in the first lane. Each lane represents a transgenic root from an individual plant. The cDNA from RNA extracted from wild type (WT) roots did not produce an amplicon for any of these genes. However, cDNA from the wild type was present, and an amplicon was produced by PCR when the cDNA was used as template with primers for a soybean control gene. RNA was extracted from three roots, individually, and independently made into cDNA. Each was examined by PCR and visualized as above. All samples from transgenic roots produced amplicons according to the appropriate Arabidopsis gene.

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