Skip to main content
Figure 5 | BMC Plant Biology

Figure 5

From: A nuclear-encoded chloroplast protein harboring a single CRM domain plays an important role in the Arabidopsis growth and stress response

Figure 5

Splicing patterns of chloroplast transcripts and rRNA processing in cfm4 mutant plants. (A) Total RNAs were extracted from 4-week-old wild-type (W) and cfm4 mutant (K), and the levels of intron-containing chloroplast transcripts were analyzed by RT-PCR. Identical results were obtained from independent experiments, and a representative result is shown. (B) Total RNA was extracted from 4-week-old wild-type (WT), cfm4 mutants, and complementation line (Com1) and was separated on a 1.2% formaldehyde agarose gel. The levels of the processed products of 23S, 16S, 5S, and 4.5S rRNAs were determined by Northern blot analysis using the probes corresponding to each gene, represented by thick lines below each gene. The relative intensities of rRNA bands in cfm4 mutants and complementation lines compared with those in wild type were calculated and values under each lane are means ± SD (n = 4).

Back to article page