Figure 4

Subunit A transcriptional response to environmental stress conditions in Arabidopsis seedlings. Each lane contained 20 μg whole RNA. The probe used was the same for organ level RNA blot analysis (1700 base pair subunit A cDNA labeled with digoxigenin). Lanes correspond to the following: N-normal, untreated control plants grown at 23°C with 16 hour photoperiod, S-sodium chloride treated plants subjected to 100 mM sodium chloride in the medium, E-etiolation, where plants were germinated in light for three days then transferred to total darkness for four days, C-cold, where plants were germinated under normal conditions for three days and then transferred to a cold room at 6°C for four days.