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Table 1 Polymerase Chain Reaction primers. Primers SM-2, SM-3 and VA-1, VA-9 were utilized in the generation of digoxigenin labeled probes for hybridization. Primers T-11 through T-42 were utilized to amplify subunit A transcripts based on the position of the poly A tail.

From: Multi site polyadenylation and transcriptional response to stress of a vacuolar type H+-ATPase subunit A gene in Arabidopsis thaliana

Primer

5' to 3' sequence

Amplification product

5' nucleotide position in cDNA

SM-2

CCTGGTGCATTTGGTTGTGGAAA

Subunit A cDNA (300 bp probe)

779

SM-3

CATCATACTAACATTGTAACCCAT

Subunit A cDNA (300 bp probe)

1055

VA-1

TCTTCGAACACACAAGCCACTTT

Subunit A cDNA (1700 bp probe)

274

VA-9

AGAGACCATGAATGAAACCCTCT

Subunit A cDNA (1700 bp probe)

1959

T-11

TTTTTTTTTTACAAAATG

Subunit A, 3' end transcript-1

2264

T-12

TGAGAGAGCAGCTGGAAT

Subunit A, 3' end transcript-1

1702

T-21

TTTTTTTTTTATCCCCAA

Subunit A, 3' end transcript-2

2227

T-22

TCAGAAGTTCGAAGACCC

Subunit A, 3' end transcript-2

1789

T-31

TTTTTTTTTTGGAGATAA

Subunit A, 3' transcript-3

2210

T-32

CTGGATTCCGTGCTTTGG

Subunit A, 3' transcript-3

1866

T-41

TTTTTTTTTTCAAATCAA

Subunit A, 3' transcript-4

2173

T-42

GGAGAAAGAGGGTTTCAT

Subunit A, 3' transcript-4

1952