Figure 5
From: Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants
Deviations from most frequent codon usage and protein consensus sequences in CYP98s from pine and Solanaceae. Comparison of the original CODEHOP sense and reverse primers (CYP98c and CYP98cr) to the primers adapted to the codon usage of pine and Solanaceae (P98n and P98nr) and to the real sequences of recently available EST or cDNAs from P. teada (CYP98Pt: AY064170), or from Solanaceae (CYP98S1-6). Alignment of available CYP98 ESTs from tomato and potato reveals several subgroups with slightly different sequences in the primer regions: CYP98S1: BG598096/BM113871/BE450893/BE451611, CYP98S2: BE436335/BE432077/BE431773/BM535273, CYP98S3: BE451666, CYP98S4: BG594285/BG594451/BG598096/BM113871, and CYP98S5: BE450893 (Solanum tuberosum accessions are shown in bold characters, L. esculentum are italicized). Sequences matching both tested primers are boxed in blue. Divergence from amino acid consensus is shown in red.