Figure 3

Microfilaments make direct contact with stromules A) DIC image of an amyloplast (p) with a thick stromule in dark-grown hypocotyl epidermis. The tip of the stromule is marked by an arrowhead in A&B. B) MFs run parallel along the sides of the stromule shown in A (arrows) and perpendicular to the stromule at its tip (arrowhead). GFP-hTalin-labeled MFs are false-colored red. The DIC image from A is false-colored green. C) DIC image of a chloroplast with stromule in a light-grown hypocotyl cell. Stromule is marked by an arrowhead in C&D. D) MFs terminate on the surface of the stromule shown in C (arrow). GFP-hTalin-labeled MFs are false-colored red. The DIC image from C is false-colored green. Chlorophyll fluorescence is false-colored blue. Bars: 5 μm.