Figure 4

Expression of rF6Hy/rF6Hys as detected by immunoblotting using anti-His antibody (A) Time course induction of rF6Hy. (B) Purification of rF6Hy on Ni-NTA. Lanes: 1, Bound 2 (2 mL eluate; 5 μg); 2, Bound 1 (1 ml eluate; 5 μg); 3, wash (10 μg); 4, flow-through (10 μg). (C) Time course induction of rF6Hys. (D) Purification of rF6Hys on Ni-NTA. Lanes: 1, Bound 3 (3 mL eluate; 2 μg); 2, Bound 2 (2 mL eluate; 2 μg); 3, Bound 1 (1 mL eluate; 5 μg); 4, wash (10 μg); 5, flow-through (10 μg).