Transgenic tomato plants that overexpress the ETO1 transgene did not show altered fruit ripening. A. Expression of the ETO1 transgene in leaves of T1 individuals. Expression of ETO1 was analyzed by RT-PCR. GAPDH was used as an internal control. One microgram of total RNA was used for each reaction. WT: wild type (Lycopersicon esculentum cv. Shu-gyoku); OE 9, OE 14-0, OE 14-1, OE 14-2 represent independent T0 transformants. Numbers under the horizontal lines represent T1 segregating individuals derived from the corresponding T0 parents. Genotyping for the ETO1 transgene was also performed by PCR and shown under the photograph. B. Representative phenotype of two independent T1 progenies of ETO1 transgenic tomato (line #14-1-5 and #9-2) and wild type (WT). Fruits were harvested at breaker stage and allowed to ripen for further days as indicated.