BMC Plant Biology

Table 4 PCR amplification profiles used for optimization of PCR conditions for each marker.

From: Development of ESTs from chickpea roots and their use in diversity analysis of the Cicergenus

Name	Profile	Temperature°C	Time min. sec	Number of cycles
65-60	Denaturation	95	3.00	1
	Touch down	94	0.20	5
		65-60	0.20
		72	0.30
	Normal	94	0.20	30
		59	0.20
		72	0.30
	Extension	72	20.0	1
60-55	Denaturation	95	3.00	1
	Touch down	94	0.20	5
		60-55	0.20
		72	0.30
	Normal	94	0.20	30
		56	0.20
		72	0.30
	Extension	72	20.0	1
55-45	Denaturation	95	3.00	1
	Touch down	94	0.20	10
		55-45	0.20
		72	0.30
	Normal	94	0.20	30
		48	0.20
		72	0.30
	Extension	72	20.0	1

Back to article page

ISSN: 1471-2229

Contact us

General enquiries: journalsubmissions@springernature.com