Figure 6

RT-PCR analysis of AsCBF gene expression in three-week old oat. Plants were incubated at +4°C for the times indicated below the lanes (m, minutes; h, hours). Total RNA was isolated from leaves and PCR reactions were run in 30 cycles using AsCBF1, AsCBF3, or AsCBF4 specific primers, as indicated to the left of the picture. Equal loading and RNA quality was controlled by amplification of the oat AsActin1 gene. A 1 kb ladder was used as a size marker (not shown).