Figure 3

Wound induced nuclear collapse in nuclear marker lines. (A) Nuclear response in line N6. Following wounding of a hypocotyl, the nucleus (arrow) first swells then contracts. The asterisk at time = 0 illustrates the normal lentoid shape of hypocotyl nuclei. Contraction is followed by a decrease in GFP fluorescence, most evident between the 16 and 20-min time points. This is followed later by intense nuclear propidium iodide staining (shown in red, adjacent to arrow). (B) Nuclear response in a wounded hypocotyl of an N7 line. Contraction of the nucleus (red arrow), decrease in nuclear fluorescence and release of nuclear fluorescence into the cytoplasm are evident. Note the formation of lobes (marked with a white arrow) around the contracting nucleus (red arrow). The time series in A and B are brightest-point reconstructions. Times shown are in min. Scale bars: A = 25 μm, B = 10 μm.