Figure 5

ER vesiculation during wound induced cell death. A wound-proximal petiole epidermal cell of ER membrane marker line Q4 was imaged. The panel shows time points (in min) after nuclear contraction. Prior to the times shown, part of the ER network formed bubble-like structures rapidly after wounding. The remaining intact tubular ER subsequently degenerated, forming large numbers of vesicular structures. Images are brightest point reconstructions of Z-series collected at 2 minutes intervals after wounding. Times shown are in minutes. Scale bar = 10 μm.