Regulation of gene expression by photosynthetic signals triggered through modified CO2availability

BMC Plant Biology

Table 1 Energetisation and phosphorylation state under influence of different acceptor availabilities: ATP content, ADP content, 3-PGA content and DHAP content in wt, adg1, pgr1 and vtc1 under different CO₂ regimes (0 ppm, 350 ppm and 2000 ppm CO₂).

			Start		0 ppm CO₂		350 ppm CO₂		2000 ppm CO₂
wt	ATP	[nmol/g FW]	97.6 ± 15.6	c	172.9 ± 31.9	a	79.1 ± 14.7	eg	60.9 ± 9.5	efg
	ADP		17.5 ± 2.8	c	29.7 ± 4.5	b	36.2 ± 10.4	b	33.1 ± 23.3	bc
	3PGA		294.0 ± 149.2	abc	117.0 ± 73.3	c	404.1 ± 44.4	b	301.0 ± 126.4	bc
	DHAP		100.0 ± 10.4	abc	71.7 ± 40.2	c	36.1 ± 15.3	b	76.4 ± 49.5	bc
	ATP/ADP		5.6		5.8		2.2		1.8
	[NADPH/NADP⁺]_calc		23.0		34.0		16.7		40.3
	Assimilatory Force		333.0		600.4		87.6		248.7
adg1	ATP	[nmol/g FW]	68.2 ± 8.7	e	149.6 ± 25.6	abc	88.8 ± 5.1	c	77.9 ± 13.4	ed
	ADP		20.6 ± 10.1	bc	113.6 ± 25.0	a	39.7 ± 24.5	bc	18.8 ± 4.2	bc
	3PGA		341.2 ± 123.3	b	113.7 ± 37.5	c	487.6 ± 81.3	ab	256.4 ± 123.3	bc
	DHAP		46.0 ± 15.0	b	54.0 ± 38.8	c	45.3 ± 24.2	ab	75.8 ± 96.2	bc
	ATP/ADP		3.3		1.3		2.2		4.2
	[NADPH/NADP⁺]_calc		16.6		63.9		16.9		25.8
	Assimilatory Force		132.1		456.7		91.0		289.5
pgr1	ATP	[nmol/g FW]	71.1 ± 12.4	ef	57.5 ± 9.3	ef	84.6 ± 4.4	d	53.4 ± 9.3	f
	ADP		24.5 ± 10.3	bc	29.5 ± 7.4	b	44.3 ± 12.7	b	24.2 ± 11.0	c
	3PGA		511.9 ± 142.0	ab	137.2 ± 49.2	c	522.5 ± 14.3	a	249.9 ± 96.8	bc
	DHAP		90.0 ± 13.0	ab	43.5 ± 27.3	c	46.1 ± 11.8	a	44.1 ± 13.4	bc
	ATP/ADP		2.9		1.9		1.9		2.2
	[NADPH/NADP⁺]_calc		22.9		44.4		18.4		28.2
	Assimilatory Force		172.3		311.0		86.5		173.0
vtc1	ATP	[nmol/g FW]	116.0 ± 21.6	bc	101.6 ± 38.0	bcdg	96.8 ± 9.8	cdg	79.9 ± 12.7	ceg
	ADP		11.9 ± 4.5	bc	74.8 ± 10.3	a	33.1 ± 14.1	b	39.2 ± 22.0	bc
	3PGA		355.1 ± 35.9	ab	144.3 ± 96.5	c	392.2 ± 130.9	b	288.3 ± 61.1	bc
	DHAP		40.0 ± 15.0	ab	56.4 ± 23.2	c	50.9 ± 24.3	b	29.0 ± 9.8	bc
	ATP/ADP		9.8		1.4		2.9		2.0
	[NADPH/NADP⁺]_calc		5.3		58.5		17.9		19.4
	Assimilatory Force		110.4		383.4		127.3		98.5

Different letters indicate significant differences. The data are means of n = 4–6, ± SE from at least 4 different experiments. ATP/ADP ratio, [NADPH]/[NADP⁺]_calc and assimilatory force F_A (according to Dietz & Heber 1989). These derived parameters were calculated from the primary data above.

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