Table 4 A list of primer sets for 44 EST microsatellite markers in the Stenogyne EST library. 35 primer sets were tested initially with Stenogyne rugosa (unigene IDs with bold text), 30 of which gave clear PCR products. Of these 30 primer pairs, 24 (marked with an asterix) gave positive amplification for four Hawaiian mint accessions.
Unigene ID | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') | Expd. product size (bp) |
|---|---|---|---|
260553* | CCCTCCGACGACACTAAA | CGACAATCTGGCAAACAG | 322 |
260567* | GAAAGTGAAAGCAGCAGATAC | ATGTGGAGGAATGATGACAC | 275 |
260570* | GTGGGAGAAACCTGAAGAG | TGTATTGACTGCCCTGATG | 109 |
260579 | TTTCTCTTCTCTCACTCACTTTC | TGATTACACCACTTCTATTGTT | 226 |
260619* | ATCTTCCCTTCCTCAAATC | TCATCATCGTCGTCTTCTTC | 325 |
260625 | GGAAGTAGAAGCAGAAGAAGG | GAGTTAGCATTGAAGGAAACA | 345 |
260641* | CAAGAATCAAACAGACCAGAC | CAGGGAAGGTAAAGCAATC | 375 |
260657* | TAAGTCTCATAGCAAGCGAAC | TATTCTACATCCCTCACACCC | 128 |
260658 | CACACCAAATCGTCAAATC | CTTCCCTCTATTCTCCAAACT | 239 |
260658 | CACACCAAATCGTCAAATC | CTTCCCTCTATTCTCCAAACT | 239 |
260664* | AACATTCACCAACCACCTT | GTCCCAGAGCACCTTCTT | 186 |
260670* | AAGAAAGAGAAGAAGGATGGT | AGAAGGGTTTGAGTGGATG | 169 |
260691* | TCCGAAATCATACTCATAAA | GGGCGATGTGCTTAGTTC | 258 |
260708* | ACCAGGAGGAAGATTGGA | TATGATTGGAGAGGTGAGATG | 217 |
260711 | GCACGAGGCAGTAATCTTT | GCAAATAATCACCACAGTTCTT | 395 |
260714* | AATAGAGATTGGAGAGAAGCA | CGACACAAACGAAACATC | 321 |
260715* | AAATCTGGGCTACTCAAACA | GGTCAGTCTTCTGTGAACCA | 190 |
260720* | ACGAGGTTTAGAAGCAAAGT | GTTGGAGTATCTGGGAAGAAG | 159 |
260754* | CACGAGGATTTACTGCTTCA | ACAAGGACATAGTTGGAGGTT | 314 |
260776* | ACTGTTGTTGATGTTCCC | TCATTCTTCCACCATTTGA | 257 |
260779 | CCTTCACCTGCTCTTCATAC | TCTTTACTCTTCTCTTTCTGACC | 219 |
260781 | GCACGAGGACAAGAAATAGA | CGCTGCCTGAAGATGAGA | 226 |
260802* | GAAAGTGAAAGCAGCAGATAC | GATGTGGAGGATGATGACAC | 270 |
260810* | GCTTCCTTCTCTTCCCAA | ACTGGTTCCGATTCTGTATG | 220 |
260811* | CAGTTGGGAAAGATAAACAGA | GGAACACAAGGAGAGGATAAA | 380 |
260853 | CGGATTGCTGATTTACTGA | GAGATTCAAACGAACACGA | 174 |
260883* | AGAAGGAGCGGGAGGTTT | TAATAATCTTGCGTCGGAG | 278 |
260903 | ACGAGAATGGGATAAAGAAAC | ATCGGTCGGTTGAGTAAGA | 362 |
260907 | CATTGGCTGGTCATAAGG | TAGTAGTGTTTGGGAGGATTG | 210 |
260915* | CATCGGTTAGAAACACAAGAA | CAAACAAAGAGAAGCCTACC | 305 |
260925 | ATAAATCTCTCCCTCCTCTCC | TTCTTCGTCGTTCCTCCT | 400 |
260939* | CGAATGTAGAAAGATAAAGGAG | CTGGACAAGTAAACGGTAGG | 221 |
260940* | GTTACATACCGTTCCCACC | TACCCTCTCTCATCCTCAAC | 394 |
260971 | TGTTGGAGTGTAAAGTTGGTT | GAGTTGTTGTTGCTGACGA | 380 |
260986 | AGAGACTGTGAGTGAGCAGG | CCATAACATCAATACCATCCA | 374 |
260996 | CCAGGGTGAACAATGAAG | TTCTACGGTTTGAGGGAAG | 165 |
261018 | ACTGGAGGAAGAAAGCAATAC | TTTACCAAATGAACCAATCC | 236 |
261039* | CATAGTAACAGATGAACCCTAA | ATCAACCCGTGAAGAAACC | 220 |
261062 | TTCTCTCACTCAATCCTGAAA | TACAACGCAATGACCGATAC | 299 |
261064* | CAACGCACACACTATGCTTC | TATTCTGGGTAGGACCATTC | 228 |
261094 | GCAGTGATGGCACAGTTAG | CGAGAAGTCGGCAGTTCA | 317 |
261109 | ATTCATTCATTCCGCTACAC | CAGAGGGCTGCTAAGATG | 157 |
261109 | ATTCATTCCGCTACACAAA | GGTTTATCCGCTGGTGGT | 366 |
261131 | ACGGATGGACTTAGGACTTT | TTTGAGATTTGAGTAGTTTGAGA | 241 |