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Figure 3 | BMC Plant Biology

Figure 3

From: The PTI1-like kinase ZmPti1a from maize (Zea maysL.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte

Figure 3

Transient expression of GFP fusion constructs. Wild type ZmPti1a, ZmPti1b, ZmPti1c, SlPti1 and N-terminal mutants of ZmPti1a were transiently expressed as C-terminal GFP fusion proteins in onion epidermal cells and in in vitro germinating pollen, respectively. Schematic representations of wild type and mutant GFP fusions are depicted in the table. Amino acids being potential targets for N-terminal modification are marked with asterisks above the ZmPti1a wild type sequence. For mutant constructs the subcellular localization is symbolized in the table; nucleus (n); cytoplasmic granules (g); cytoplasm (c). (A) Wild type ZmPti1a. Top panel: onion epidermal cell; bottom panel: pollen tube (B) 24 aa of the ZmPti1a N-terminus fused to GFP. (C) N-terminally truncated ZmPti1a. Top panel: onion epidermal cell; bottom panel: pollen tube (D) Wild type SlPti1 from tomato. (E) GFP control. (F) ZmPti1a containing Cys3 → Ala3 mutation. (G) ZmPti1a containing Gly2 → Ala2 and Cys3 → Ala3 mutation. (H) Wild type ZmPti1b. (I) Wild type ZmPti1c. Scale bars = 50 μm.

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BMC Plant Biology

ISSN: 1471-2229

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